Prabhakara Rao Dasari; Sofia Pilli; Ravi Jon
Abstract
The present work reports an ecofriendly method for biosynthesis of AgNPs using an aqueous leaves extracts of Carissa carandas and Nerium indicum plants. These extracts play a significant role for the formation of AgNPs. The obtained AgNPs were characterized by Ultraviolet–Visible Spectrophotometry. ...
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The present work reports an ecofriendly method for biosynthesis of AgNPs using an aqueous leaves extracts of Carissa carandas and Nerium indicum plants. These extracts play a significant role for the formation of AgNPs. The obtained AgNPs were characterized by Ultraviolet–Visible Spectrophotometry. The UV-spectrum showed that the absorbance peak at 440 nm. TEM analysis shown morphology and crystalline of nanoparticles as 20 and 40 nm sizes and spherical shape. The SEM and EDXA analysis was shown identify elemental composition and confirm the NPs of desired elements. The FTIR analysis showed functional group elements as amide, hydroxyl and amino groups in NPs. These results were confirming the AgNPs have been best activity against human pathogens. The AgNPs showed high potential antibacterial activity toward the selected pathogenic bacteria. Therefore, Carissa carandas and Nerium indicum leaves aqueous extract AgNPs can provide application in future development as nano-medicine, nanotechnology as antifungal activity on Aspergillus niger and candida albicans and then anticancer activity on MCF-7 cell line. Finally, the Nerium indicum leaves aqueous extract AgNPs were more effective in antibacterial, antifungal and anticancer activity than Carissa carandas AgNPs.
Alireza Ebrahiminezhad; Saeed Taghizadeh; Aydin Berenjian; Amid Rahi; Younes Ghasemi
Abstract
For the first time, green synthesis of silver nanoparticles was developed by treating Ag + ions with Zataria multiflora leaf extract. Leaf extract quantity, AgNO3 concentration and reaction temperature were determined as significant factors in the bioreduction reaction. Carbohydrates of the leaf extract ...
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For the first time, green synthesis of silver nanoparticles was developed by treating Ag + ions with Zataria multiflora leaf extract. Leaf extract quantity, AgNO3 concentration and reaction temperature were determined as significant factors in the bioreduction reaction. Carbohydrates of the leaf extract were identified as the effective compound for reduction and capping of AgNPs. Also, TEM micrographs illustrated that micro and nano scale carbon-based materials act as structural scaffold for nucleation and growth of particles. Oxygen-bearing functional groups were identified as active groups for reduction of Ag + ions. The prepared particles were spherical from 16.3 nm to 25.4 nm with mean particles size of 20.3 nm.
Aftab H. Mondal; Mudsser Azam; Mohammad T. Siddiqui; Qazi M. Rizwanul Haq
Abstract
In the present study extracellular synthesis of silver nanoparticles (AgNPs) was achieved using cultural supernatant of bacterial isolate Aeromonas dhakensis AS3. Biosynthesis of AgNPs was completed within 120 min by incubating cell free supernatant with silver nitrate solution under illumination. Brown ...
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In the present study extracellular synthesis of silver nanoparticles (AgNPs) was achieved using cultural supernatant of bacterial isolate Aeromonas dhakensis AS3. Biosynthesis of AgNPs was completed within 120 min by incubating cell free supernatant with silver nitrate solution under illumination. Brown color appearance of solution due to surface plasmon resonance (SPR) and absorption maxima centered at 405 nm was indicated formation of AgNPs. Fourier transform infrared spectroscopy (FTIR) spectrum analysis revealed the presence and association of possible biomolecules with AgNPs during synthesis. Atomic force microscopy (AFM), Field emission scanning electron microscopy (FE-SEM) and High resolution transmission electron microscopy (HR-TEM) showed spherical nanoparticles with an average size of 5 nm. X-ray diffraction (XRD) and Energy Dispersive X-ray (EDX) spectrum confirmed crystallinity and purity of AgNPs. The synthesized AgNPs was found to have significantly independent as well as combined activity against multidrug resistant extended spectrum β-lactamases (ESBLs) producing Acinetobacter junii, E.coli and Klebsiella spp. harboring TEM and/or CTX-M genes. This work demonstrates the possible use of biosynthesized AgNPs to combat ESBLs producing pathogens.
Shadakshari Sandeep; Arehalli S. Santhosh; Ningappa Kumara Swamy; Gurukar S. Suresh; Jose S. Melo; Puttaswamappa Mallu
Abstract
In the present work, we report on the biosynthesis of silver nanoparticles (AgNPs) using leaf extract of Convolvulus pluricaulis (Shankapushpi, bindweed) at room temperature. Synthesis of AgNPs is carried out by incubating the leaf extract in presence of AgNO3. Formation of AgNPs is confirmed by the ...
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In the present work, we report on the biosynthesis of silver nanoparticles (AgNPs) using leaf extract of Convolvulus pluricaulis (Shankapushpi, bindweed) at room temperature. Synthesis of AgNPs is carried out by incubating the leaf extract in presence of AgNO3. Formation of AgNPs is confirmed by the appearance of a prominent surface plasmon resonance band in the UV-visible spectrum at 420 nm. The biosynthesized AgNPs are characterized by powder X-ray diffraction (XRD) studies, Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), thermo gravimetric analysis (TGA) and differential thermo gravimetric (DTG) analysis. Further, the biosynthesized AgNPs are investigated for their catalytic, electrocatalytic and phenol remediation properties. The investigations revealed that the biosynthesized AgNPs excel in their respective applications. Based on the results, present study concludes that AgNPs can be biosynthesized using leaf extract of Convolvulus pluricaulis and further can be employed for applications in electrochemical sensing, dye degradation and phenol remediation.
Anal K. Jha;Kamal Prasad
Abstract
Biosynthesis, characterizations and applications of nanoparticles have become an important branch of nanotechnology nowadays. In this paper, green synthesis of silver nanoparticles (AgNPs) using the alcoholic extract of Clerodendron infortunatum as a reducing and stabilizing agent, has been discussed. ...
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Biosynthesis, characterizations and applications of nanoparticles have become an important branch of nanotechnology nowadays. In this paper, green synthesis of silver nanoparticles (AgNPs) using the alcoholic extract of Clerodendron infortunatum as a reducing and stabilizing agent, has been discussed. This biosynthetic method is simple, cost-effective and reproducible. Formation of AgNPs was established by X-ray diffraction, scanning and transmission electron microscopy, UV-visible spectroscopy techniques. The phytochemicals responsible for nano-transformation were principally phenolics, tannins and organic acids present in the leaves. Further, AgNPs were used for antibacterial treatment of cotton fabrics which was tested by antibacterial activity assessment of textile material by agar diffusion method against Staphylococcus aureus. The colloidal AgNPs have been soaked onto cotton fabrics and studied for their effective antibacterial activity toward Staphylococcus aureus which showed remarkable antibacterial activity.
Bandita Mohapatra; Reena Kaintura; Jaspal Singh; Sini Kuriakose; Satyabrata Mohapatra
Abstract
Stable aqueous dispersions with high concentration of silver nanoparticles were synthesized by a facile and green synthetic route by treating silver ions with aqueous Citrus limon extract, used as a reducing and capping agent. The formation and growth of silver nanoparticles, prepared by this simple ...
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Stable aqueous dispersions with high concentration of silver nanoparticles were synthesized by a facile and green synthetic route by treating silver ions with aqueous Citrus limon extract, used as a reducing and capping agent. The formation and growth of silver nanoparticles, prepared by this simple and convenient method, was monitored using UV-visible absorption spectroscopy. The effects of Ag concentration, Citrus limon extract concentration, in-situ and ex-situ pH variations upon NaOH addition on the structural, optical and plasmonic properties of the synthesized Ag nanoparticles were investigated. X-ray diffraction studies revealed the formation of Ag nanoparticles, whose morphology was studied using atomic force microscopy. UV-visible absorption studies revealed surface plasmon resonance (SPR) peak around 465 nm, confirming the presence of Ag nanoparticles. The SPR peak blue shifted along with significant enhancement in intensity with increase in Ag concentration and pH, due to the growth and increased aggregation of Ag nanoparticles. We have shown that addition of NaOH is a key to rapid biosynthesis of stable aqueous dispersions of high concentration of silver nanoparticles. This green synthetic route provides faster synthesis of silver nanoparticles with improved colloidal stability, which can be used in foods, cosmetics and biomedical applications.
Akl M. Awwad; Borhan Albiss
Abstract
Copper hydroxide nanowires were synthesized from copper chloride dihydrate and sodium hydroxide in the presence of Pistachio leaf extract at room temperature. The results of scanning electron microscopy (SEM) exhibited that the copper hydroxide Cu(OH)2 are nanowires ...
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Copper hydroxide nanowires were synthesized from copper chloride dihydrate and sodium hydroxide in the presence of Pistachio leaf extract at room temperature. The results of scanning electron microscopy (SEM) exhibited that the copper hydroxide Cu(OH)2 are nanowires in shape and having an average diameter of ca. 10 nm and lengths of up to 500 nm . The Fourier transfor infrared spectroscopy (FT-IR) results examined the bioactive functional groups , which acts as dispersing, binding and capping agent for the copper hydroxide nanowires. X-ray diffraction (XRD) spectra confirmed the copper hydroxide nanoparticles crystalline in nature. The current research introduces a new, simple, and rapid route for the in –laboratory synthesis of copper hydroxide nanowires. This facile and green approach may provide a useful tool to large scale synthesis other copper nanoparticles that have potential biotechnology.
Akl M. Awwad; Nida M. Salem; Ammany O. Abdeen
Abstract
This paper reports a rapid and eco-friendly green method for synthesis of silver nanoparticles from silver nitrate solution using loquat leaf extract. Effect the amount of leaf extract, reaction time, silver nitrate concentration and temperature were investigated. Biosynthesized silver nanoparticles ...
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This paper reports a rapid and eco-friendly green method for synthesis of silver nanoparticles from silver nitrate solution using loquat leaf extract. Effect the amount of leaf extract, reaction time, silver nitrate concentration and temperature were investigated. Biosynthesized silver nanoparticles (AgNPs) were characterized by X-ray diffraction (XRD), atomic absorption spectroscopy (AAS) and Fourier transform infrared spectroscopy (FT-IR). UV-vis spectroscopy showed that the surface plasmon resonance (SPR) at 425 nm. The structural peaks in XRD pattern and average crystalline size around 18 nm clearly illustrates that AgNPs synthesized by our green method were nanocrystalline in nature with face centered cubic geometry. The antibacterial activity of biosynthesized silver nanoparticles showed effective inhibitory activity against water borne pathogens, Shegella and Listeria bacteria.
Laura Christensen; Singaravelu Vivekanandhan; Manjusri Misra; Amar Kumar Mohanty
Abstract
Biological synthesis of silver nanoparticles using Murraya koenigii leaf extract was investigated and the effect of broth concentration in reduction mechanism and particle size is reported. The rapid reduction of silver (Ag + ) ions was monitored using UV-visible spectrophotometry and showed formation ...
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Biological synthesis of silver nanoparticles using Murraya koenigii leaf extract was investigated and the effect of broth concentration in reduction mechanism and particle size is reported. The rapid reduction of silver (Ag + ) ions was monitored using UV-visible spectrophotometry and showed formation of silver nanoparticles within 15 minutes. Transmission electron microscopy (TEM) and atomic force microscopy (AFM) analysis showed that the synthesized silver nanoparticle are varied from 10-25 nm and have the spherical shape. Further, the XRD analysis confirms the nanocrystalline phase of silver with FCC crystal structure. From this study, it was found that the increasing broth concentration increases the rate of reduction and decreases the particle size.
Ratnika Varshneya; Seema Bhadauriaa; Mulayam S.Gaur
Abstract
This work reports a novel biological method for the synthesis of rod shaped silver nanoparticles by exploiting sundried Stevia rebaudiana leaves at ambient conditions. On treatment of aqueous solutions of silver with leaf powder, not only the rod shaped silver nanoparticles ranging from 80-200 nm diameter ...
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This work reports a novel biological method for the synthesis of rod shaped silver nanoparticles by exploiting sundried Stevia rebaudiana leaves at ambient conditions. On treatment of aqueous solutions of silver with leaf powder, not only the rod shaped silver nanoparticles ranging from 80-200 nm diameter and 400-800 nm height, but also cubes ranging from 55 to 80 nm in size, could be rapidly fabricated. The rate of reduction is much faster than those observed in earlier studies, highlighting the possibility that biological methodologies will achieve rates of synthesis comparable to those of chemical methods. The approach also appears to be a cost-efficient alternative to conventional methods, so it would be suitable for developing a biological process for large scale production. Instead of the boiled leaf broth used in previous studies, sundried leaf biomass could be preserved as an excellent bio-reductant, conveniently available any time for biosynthesis of the nanoparticles.
