The present work reports an ecofriendly method for biosynthesis of AgNPs using an aqueous leaves extracts of Carissa carandas and Nerium indicum plants. These extracts play a significant role for the formation of AgNPs. The obtained AgNPs were characterized by Ultraviolet–Visible Spectrophotometry. The UV-spectrum showed that the absorbance peak at 440 nm. TEM analysis shown morphology and crystalline of nanoparticles as 20 and 40 nm sizes and spherical shape. The SEM and EDXA analysis was shown identify elemental composition and confirm the NPs of desired elements. The FTIR analysis showed functional group elements as amide, hydroxyl and amino groups in NPs. These results were confirming the AgNPs have been best activity against human pathogens. The AgNPs showed high potential antibacterial activity toward the selected pathogenic bacteria. Therefore, Carissa carandas and Nerium indicum leaves aqueous extract AgNPs can provide application in future development as nano-medicine, nanotechnology as antifungal activity on Aspergillus niger and candida albicans and then anticancer activity on MCF-7 cell line. Finally, the Nerium indicum leaves aqueous extract AgNPs were more effective in antibacterial, antifungal and anticancer activity than Carissa carandas AgNPs.