Document Type : Research Article
Authors
- Ryan M Lumod 1
- Khia Jane D Avila 2
- Rolen Brian P Rivera 3, 1
- Miceh Rose A Magdadaro 3, 4
- Noel Lito B Sayson 5, 1
- Felmer S Latayada 6
- Gerard G Dumancas 7
- Rey Y Capangpangan 8
- Arnold C Alguno 1
1 Research Center for Energy Efficient Materials (RCEEM), Premier Research Institute of Science and Mathematics (PRISM), Mindanao State University – Iligan Institute of Technology, A. Bonifacio Avenue, Iligan City, 9200 Philippines
2 Research Center for Energy Efficient Materials (RCEEM), Premier Research Institute of Science and Mathematics (PRISM), Mindanao State University – Iligan Institute of Technology, A. Bonifacio Avenue, Iligan City, 9200 Philippines
3 Department of Physics, Mindanao State University – Iligan Institute of Technology, A. Bonifacio Avenue, Iligan City, 9200 Philippines,
4 Mindanao State University, Lanao del Norte Agricultural College, Lanao del Norte, Philippines
5 Department of Physics, Mindanao State University – Iligan Institute of Technology, A. Bonifacio Avenue, Iligan City, 9200 Philippines
6 Department of Chemistry, Caraga State University, Butuan City 8600, Philippines
7 Department of Chemistry, Loyola Science Center, The University of Scranton, Scranton, Pennsylvania 18510, United States of America
8 Department. of Physical Science & Mathematics, Mindanao State University at Naawan, Naawan Misamis Oriental, 9023 Philippines
Abstract
Gold nanoparticles (AuNPs) have wide-ranging applications across scientific disciplines and industries. However, its conventional synthesis methods pose environmental and health risks, prompting the rise of green chemistry for sustainable and eco-friendly nanoparticle production. Plant extracts rich in bioactive compounds capable of reducing and capping nanoparticles have emerged as promising alternatives. Among these sources, Aloe vera, renowned for its diverse phytochemicals, presents an attractive avenue for nanoparticle synthesis devoid of hazardous reagents. This study delves into the one-step green synthesis of AuNPs employing aloe vera extract and examines their antibacterial efficacy against Gram-positive and Gram-negative bacteria. The synthesized AuNPs exhibited a reddish-purple color with localized surface plasmon resonance peaks at 529 nm, 535 nm, and 541 nm, corresponding to varying gold precursor concentrations (0.1 mM, 0.3 mM, and 0.5 mM). FTIR analysis confirmed the presence of bioactive compounds involved in the reduction and capping of AuNPs. Characterization via Transmission Electron Microscopy showed spherical AuNPs ranging from 10 nm to 39 nm in diameter, with stability indicated by zeta potential values of -37.3 mV, -28.7 mV, and -24.7 mV for the respective concentrations. Notably, AV-AuNPs demonstrated significant antibacterial activity, with inhibition zones of 34 mm against E. coli and 18 mm against B. subtilis, attributed to their ability to penetrate bacterial membranes and induce cell lysis.
Graphical Abstract
)
Keywords
Main Subjects
)