A nanoscale optical fiber probe with proper tailored tip diameter, taper angle and taper length is essential to monitor biological phenomena in single cells. In this work, F-MCC-T optical fiber, a multimode optical fiber with good propagating property within UV-Vis range is selected to fabricate the nanoprobe by pulling method. The method, particularly the factors affecting the tip diameter, taper angle and the taper length were thoroughly studied. Results show that optimal conditions could produce a nanoscale probe with a 50 nm of diameter, 6000 nm of taper length and taper angle of 28 degree with good reproducibility.