Seetharamaiah Nalini; Seetharamaiah Nandini; Gurukar Shivappa Suresh; Jose Savio Melo; Shivayogeeswar E. Neelagund; Hunasepalya Nagaiah Naveen Kumar; Jakkid Sanetuntikul; Sangaraju Shanmugam
Abstract
In this article, we report the pre-screening of anti-cancer effect of Calotropis procera against glioblastoma cell lines (LN-18) by means of electrochemical methods. Soxhlet assisted extraction (SAE) has been employed to extract the polyphenol contents present in the leaf of Calotropis procera. The phytochemical ...
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In this article, we report the pre-screening of anti-cancer effect of Calotropis procera against glioblastoma cell lines (LN-18) by means of electrochemical methods. Soxhlet assisted extraction (SAE) has been employed to extract the polyphenol contents present in the leaf of Calotropis procera. The phytochemical analysis of the extract has been studied and the polyphenol contents were determined using Folin Ciocalteau method. To study the anticancer effects of the aqueous plant extract, a cytosensor (Gr/NT-G/LN-18) was fabricated and its possible mechanism for DNA binding was studied using graphite/ poly(allylamine hydrochloride)/nanotube-graphene composite /polypyrrole /de-oxy ribonucleic acid (Gr/PAH/NT-G/PPy/DNA) modified electrode. The electrochemical characteristics of the proposed Gr/NT-G/LN-18 cytosensor towards the plant extract were evaluated using electrochemical techniques like cyclic voltammetry and differential pulse voltammetry. Scanning electron microscopy (SEM) and energy dispersive analysis of x-ray (EDAX) have been employed to study the physical characterization of the Gr/PAH/NT-G/PPy/DNA modified electrode. These results indicate that the plant extract has an ability to act as an antiglioblastoma against LN-18 cancer cells.
Bishnu K. Pandey; Ashutosh K. Shahi; Nitisha Srivastava; Girjesh Kumar; Ram Gopal
Abstract
In the present study we have successfully synthesized cobalt and cobalt oxide NPs in ethanol and double distilled water respectively. Structural and optical characterizations have been performed by X-ray diffraction and UV-Visible absorption spectroscopy respectively. Magnetic characterization has been ...
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In the present study we have successfully synthesized cobalt and cobalt oxide NPs in ethanol and double distilled water respectively. Structural and optical characterizations have been performed by X-ray diffraction and UV-Visible absorption spectroscopy respectively. Magnetic characterization has been performed by vibrating sample magnetometer (VSM). Particle shape and size have been estimated by transmission electron microscopy (TEM). We have studied the effect of cobalt and cobalt oxide NPs on mitotic division of meristamatic roots of Sesbania cannabina. It has been found that cobalt oxide nanoparticles were more toxic than cobalt nanoparticles and both induced various types of chromosomal aberrations in meristamatic root tip cells of sesbania cannabina.
Nuo Zhang; Caixia Xu; Qin Wei; Bin Du; Ru Li; Tianguo Zhang; Dan Wu; Yuxue Dai
Abstract
Nano-scale hydroxyapatite (HAp) was prepared by combining co-precipitation with microemulsion method, which exhibited strong adsorption for DNA due to its excellent biocompatibility and particular adsorbability. DNA and HAp could be modified onto glassy carbon electrode (GCE) by the simple and convenient ...
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Nano-scale hydroxyapatite (HAp) was prepared by combining co-precipitation with microemulsion method, which exhibited strong adsorption for DNA due to its excellent biocompatibility and particular adsorbability. DNA and HAp could be modified onto glassy carbon electrode (GCE) by the simple and convenient “tip-coating” method. Cyclic voltammetry was used to investigate the interaction of DNA immobilized on the HAp film with vitamin B12 (VB12). The existence of DNA led to the decrease of reduction current of VB12. Both the electron transfer coefficient (α) and the standard rate constant (ks) were different obtained on GCE and dsDNA/HAp/GCE, which indicated the formation of an electrochemical inactive super molecular complex DNA-nVB 12 . The equilibrium constant of this complex was calculated to be 5.35 × 10 5 mol·L -1 and the binding number between DNA and VB12 of the complex were determined to be one.
