Bindu Mangla; Sudheesh K. Shukla; Jagriti Narang; Nitika Thakur; Chaudhery Mustansar Hussain
Abstract
In current pandemic, as there is no approved specific antiviral agent for coronavirus disease (COVID-19) available till to date; Indian Ayurveda has emerged out as effective tool to control the prevailing pandemic at initial stage mainly by gearing up the immune system. Convalescent plasma therapy (CPT) ...
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In current pandemic, as there is no approved specific antiviral agent for coronavirus disease (COVID-19) available till to date; Indian Ayurveda has emerged out as effective tool to control the prevailing pandemic at initial stage mainly by gearing up the immune system. Convalescent plasma therapy (CPT) has shown a glimpse of hope and some case study supported the hypothesis that CPT could be a prospective treatment option. The current study reported first time on a real time case study in India along with the outcome that CPT could be potentially improved the treatment of severe COVID-19. The CPT results presented here have shown that clinical symptoms were significantly improved along with increase of oxyhemoglobin saturation, also increased lymphocytes count whereas decreased C-reactive proteins within 3 days without any severe adverse side effect.
Jagriti Narang; Chaitali Singhal; Vibhor Kaushal; C. S. Pundir
Abstract
Prussian Blue nanocubes / carbon nanospheres (PB-CNS) heterostructure composed of perfectly cube and spherical composite on indium tin oxide (ITO) surface were developed for in vitro sensing of anti-diabetic drug i.e. Sitagliptin (STA). Detailed morphological, electrochemical, structural and optical ...
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Prussian Blue nanocubes / carbon nanospheres (PB-CNS) heterostructure composed of perfectly cube and spherical composite on indium tin oxide (ITO) surface were developed for in vitro sensing of anti-diabetic drug i.e. Sitagliptin (STA). Detailed morphological, electrochemical, structural and optical characterization of PB-CNS/ITO electrode was done using DLS, SEM, EIS, CV. The sensor showed rapid response time (within 5 s) and the linear range from 0.001 to 10 mM with a shelf life of about 10 weeks under refrigerated conditions. We have also attempted to employ this electrode for assessment of STA in urine samples. The developed sensor exhibited high reproducibility and good storage stability.
Jagriti Narang; Nitesh Malhotra; Chaitali Singhal; C. S. Pundir
Abstract
An electrochemical biosensor based on xanthine oxidase (XOx), titanium dioxide nanoparticles and carboxylated multi-walled carbon nanotubes (TiO2/c-MWCNT) nano-composites for sensitive detection of xanthine has been developed. TiO2/MWCNT nano-composites were used as the sensing platform in order to immobilize ...
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An electrochemical biosensor based on xanthine oxidase (XOx), titanium dioxide nanoparticles and carboxylated multi-walled carbon nanotubes (TiO2/c-MWCNT) nano-composites for sensitive detection of xanthine has been developed. TiO2/MWCNT nano-composites were used as the sensing platform in order to immobilize XOx and magnify the sensor response. FTO electrode was employed to amplify electrochemical signal in the buffer solution. Detailed morphological, electrochemical, structural and optical characterization of XOx/TiO2-NPs/c-MWCNT/FTO electrode was done using XRD, DLS, SEM, EIS, CV and shows quick response time (within 30s), linearity as 0.5- 500 µM, lower detection value of 0.05 micromolar with signal: noise ratio of 3, excellent reproducibility, high selectivity and shelf life of about 8 weeks under refrigerated conditions. The developed biosensor was further used to determine the xanthine levels in the labeo fish samples obtained from market. The accuracy of the developed biosensor was cross-checked by the customary enzymic colorimetric method (99% correlation). Thus, the existing research confirms the development of a highly sensitive, stable and a reliable bio-sensing method to detect the freshness of fish samples.
Jagriti Narang; Nidhi Chauhan; Ashish Mathur; Vivek Chaturvedi; C.S. Pundir
Abstract
In present work, we employed gold nanorods for electrochemical sensing of bilirubin. A new method is developed by using covalently immobilized bilirubin oxidase (BOx) on gold nanorods and employed gold microelectrode. The sensing interface materials were characterized by dynamic light scattering (DLS), ...
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In present work, we employed gold nanorods for electrochemical sensing of bilirubin. A new method is developed by using covalently immobilized bilirubin oxidase (BOx) on gold nanorods and employed gold microelectrode. The sensing interface materials were characterized by dynamic light scattering (DLS), scanning electron microscopy (SEM), electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). The optimum response was observed at pH 7 and 35 ºC. The linear working range of the biosensor is 0.01 -500 µM. Fabricated sensing technology showed good evaluation parameters like precision (2.2 % and 3.2 %) and analytical recovery (98.2 % and 97.4 %). Bilirubin biosensor can be employed for early detection of bilirubin in blood serum to check jaundice,hyperbilirubinia and physiologic jaundice in infants.
Jagriti Narang; Utkarsh Jain; Nitesh Malhotra; Sandeep Singh; Nidhi Chauhan
Abstract
An amperometric lysine biosensor was fabricated by immobilizing lysine oxidase onto core shell magnetic nanoparticles (Core–shell MNPs)/multiwalled carbon nanotube (MWCNT) layer deposited on Au electrode via carbodiimide linkage. Transmission electron microscopy (TEM) for core–shell MNPs, ...
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An amperometric lysine biosensor was fabricated by immobilizing lysine oxidase onto core shell magnetic nanoparticles (Core–shell MNPs)/multiwalled carbon nanotube (MWCNT) layer deposited on Au electrode via carbodiimide linkage. Transmission electron microscopy (TEM) for core–shell MNPs, scanning electron microscopy (SEM), electrochemical impedance spectroscopy (EIS) and Fourier transform impedance spectroscopy (FTIR) studies were used to characterize the modified electrode. Sensor showed optimal response within 2s at 30ºC in 0.05 M sodium phosphate buffer pH 6.0 when polarized at +0.2 V vs. Ag/AgCl. Linear working range of the biosensor was determined by 0.05 -700 μM with a detection limit of 0.05 μM. A good correlation (r = 0.98) was obtained between serum lysine levels measured by the standard HPLC method (y) and the present method (x). A number of serum substances had practically no interference. The sensor was used in 150 assays and had a storage life of 180 days at 4 o C. This nanohybrid biosensor will be useful for detection of lysine in food and pharmaceutical industries.
Jagriti Narang; Nitesh Malhotra; Nidhi Chauhan; Sandeep Singh; Gajendra Singh; C.S. Pundir
Abstract
A polyphenol oxidase (PPO) was immobilized by employing magnetic nanoparticles-zinc oxide/zinc hexacyanoferrate (Fe3O4NP-ZnO/ZnHCF) hybrid film electrodeposited on the surface of Pt electrode. The surface functionalization of Fe3O4NP-ZnO/ZnHCF hybrid film was characterized by cyclic voltammetry (CV), ...
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A polyphenol oxidase (PPO) was immobilized by employing magnetic nanoparticles-zinc oxide/zinc hexacyanoferrate (Fe3O4NP-ZnO/ZnHCF) hybrid film electrodeposited on the surface of Pt electrode. The surface functionalization of Fe3O4NP-ZnO/ZnHCF hybrid film was characterized by cyclic voltammetry (CV), scanning electron microscopy (SEM), atomic force microscopy (AFM) and electrochemical impedance spectroscopy (EIS) before and after immobilization of PPO. The biosensor exhibited optimum response within 4s at pH 7.0 and 35°C and linearity in the range 0.04 to 10000 μM for acetaminophen with a detection limit of 0.04 μM (S/N=3). Accuracy of the proposed sensor was found to be 99%. The use of Fe3O4NP/ZnO/ZnOHCF for construction of amperometric acetaminophen biosensor has resulted into relatively rapid response, higher sensitivity, broad linear range, lower detection limit, good reproducibility and long term stability of this biosensor. This sensing interface provides better avenue for the fabrication of various sensor.