In present work, we employed gold nanorods for electrochemical sensing of bilirubin. A new method is developed by using covalently immobilized bilirubin oxidase (BOx) on gold nanorods and employed gold microelectrode. The sensing interface materials were characterized by dynamic light scattering (DLS), scanning electron microscopy (SEM), electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). The optimum response was observed at pH 7 and 35 ºC. The linear working range of the biosensor is 0.01 -500 µM. Fabricated sensing technology showed good evaluation parameters like precision (2.2 % and 3.2 %) and analytical recovery (98.2 % and 97.4 %). Bilirubin biosensor can be employed for early detection of bilirubin in blood serum to check jaundice,hyperbilirubinia and physiologic jaundice in infants.