Hediyeh Nejati Rad; Aliasghar Behnamghader; Mojgan Bagheri; Masoud Mozafari
Abstract
In this study, mesoporous bioactive glass 77S was synthesized by sol-gel method using two different ionic and nonionic surfactants. Physical-chemical properties of synthesized Bioglass were studied using techniques include X-ray diffraction, scanning electron microscopy, nitrogen adsorption and Fourier ...
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In this study, mesoporous bioactive glass 77S was synthesized by sol-gel method using two different ionic and nonionic surfactants. Physical-chemical properties of synthesized Bioglass were studied using techniques include X-ray diffraction, scanning electron microscopy, nitrogen adsorption and Fourier transform infrared spectroscopy. The results showed that using nonionic and ionic surfactants increased specific surface area by 3 and 5 times, respectively. By determining the silicon release in three different temperatures, the activation energy values of Si-O-Si for sample with surface area of 161 and 94 m 2 /g were calculated 1/10 and 1/5 of control glass with surface area of 34 m 2 /g. The pH of sample was evaluated in solutions buffered with TRIS. pH analysis results suggested a direct relation between surface area and pH changes, the greatest increase in pH was observed in the sample with the highest surface area. Moreover, the in vitro bioactivity test was also conducted in simulated body fluid (SBF) and formation of apatite layer was evaluated by scanning electron microscopy and X-ray diffraction after a day.
Amin Nakhi; Monireh Ganjali; Haji Shirinzadeh; Ali Sedaghat Ahangari Hossein Zadeh; Masoud Mozafari
Abstract
In this study, at first fluorapatite nanopowder (nfAp) was initially synthesized by sol-gel method and then deposited on Titanium alloys (Ti-6Al-4V) using laser cladding technique. X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) equipped ...
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In this study, at first fluorapatite nanopowder (nfAp) was initially synthesized by sol-gel method and then deposited on Titanium alloys (Ti-6Al-4V) using laser cladding technique. X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) equipped with electron dispersive spectroscopy (EDX), were applied to study the crystallite and particle size, phase and chemical structure and microstructure the powder and coating sample. The results of XRD analysis and FTIR showed the presence of fluorapatite phases and ions replacement of F with OH in the structure of apatite. The MTT cell viability assays were used to study the biocompatibility of the coating samples. The average size of the crystallites estimated from XRD patterns using the Scherrer equation was 44 nm. The prepared nfAp coating deposited on Ti6Al4V showed well-behaved biocompatibility properties.
Farnaz Ghorbani; Hanieh Nojehdehyan; Ali Zamanian; Mazaher Gholipourmalekabadi; Masoud Mozafari
Abstract
There have been several attempts to synthesis biodegradable polymeric constructs with adequate porous structures for soft connective tissues. In this study, randomly-oriented PLGA-gelatin nanofibrous scaffolds were synthesized by electrospinning method. We offered an appropriate solvent (2, 2, 2-trifluoroethanol) ...
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There have been several attempts to synthesis biodegradable polymeric constructs with adequate porous structures for soft connective tissues. In this study, randomly-oriented PLGA-gelatin nanofibrous scaffolds were synthesized by electrospinning method. We offered an appropriate solvent (2, 2, 2-trifluoroethanol) to dissolve both polymers for achieving a homogenous solution without inducing any toxic effects. The results confirmed the formation of high porous and bead free scaffolds, in which an increase in the injection rate slightly decreased the mechanical, swelling ratio and biodegradation behaviors. The modulus and tensile strength for the scaffolds with the injection rate of 0.2 ml/hr were 0.72 ±0.02 and 2.70 ±0.33, respectively. In addition, the evaluation of cell proliferation demonstrated that L929 fibroblast cells spread well on the scaffolds, indicating that they are able to support cell attachment. A possible chemical bond formation has been also suggested for the blending mixture of PLGA and gelatin molecules.
Mazaher Gholipourmalekabadi; Masoud Mozafari; Mojgan Bandehpour; Marzieh Sameni; Hossein Ghanbarian
Abstract
In this study, the effects of ethanol treatment on the mechanical and biological characteristics of the nanofibrous silk fibroin (NSF) scaffolds were evaluated. The results obtained from the mechanical tests confirmed that ethanol treatment significantly enhanced the physical properties of the scaffolds ...
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In this study, the effects of ethanol treatment on the mechanical and biological characteristics of the nanofibrous silk fibroin (NSF) scaffolds were evaluated. The results obtained from the mechanical tests confirmed that ethanol treatment significantly enhanced the physical properties of the scaffolds through the formation of a ß-sheet structure. It was shown that the ethanol treatment increased the mechanical property and cell viability, while decreased the porosity of the randomly arranged uniform nanofibers. The ultimate tensile strength for the NSF and ethanol-treated NSF (ET-NSF) scaffolds were 0.76 and 1.33 MPa, respectively. In addition, the ethanol treatment positively affected the proliferation rate of rat bone-marrow stromal cells (rBMSCs) without any detectable cytotoxicity. All the results obtained from this study strongly indicated the efficacy of ethanol treatment in enhancement of mechanical and biological characteristics of silk fibroin nanofibrous scaffolds.
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