In this study, at first fluorapatite nanopowder (nfAp) was initially synthesized by sol-gel method and then deposited on Titanium alloys (Ti-6Al-4V) using laser cladding technique. X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) equipped with electron dispersive spectroscopy (EDX), were applied to study the crystallite and particle size, phase and chemical structure and microstructure the powder and coating sample. The results of XRD analysis and FTIR showed the presence of fluorapatite phases and ions replacement of F with OH in the structure of apatite. The MTT cell viability assays were used to study the biocompatibility of the coating samples. The average size of the crystallites estimated from XRD patterns using the Scherrer equation was 44 nm. The prepared nfAp coating deposited on Ti6Al4V showed well-behaved biocompatibility properties.