Tao Yan; Dong Wei; Xianpeng Zheng; Xiaodong Xin; Nuo Zhang; Dan Wu; Liangguo Yan; He Li; Bin Du; Qin Wei
Abstract
For biomimetic synthesis of hydroxyapatite by simulating biomineralization, the polyamide 66 (PA 66) was used as biotemplate carrier, and the biotemplate was prepared by the adsorption of AOT surfactants at interfaces of polymer fasciculus. Simulating biomineralization was carried out at physiological ...
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For biomimetic synthesis of hydroxyapatite by simulating biomineralization, the polyamide 66 (PA 66) was used as biotemplate carrier, and the biotemplate was prepared by the adsorption of AOT surfactants at interfaces of polymer fasciculus. Simulating biomineralization was carried out at physiological condition (pH = 7.4, T = 37 o C). The phase components, morphologies and possible growth mechanism of calcium phosphate were studied by fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM) and scanning electron microscopy (SEM). The results indicated that the course of crystal growth, as well as the morphology and composition of product was markedly dependent on biotemplate; the crystals size were within nanometer scope, with a sphere and short rod-like shape extraordinary close to natural bone. The possible mechanism of crystal growth was discussed.
Nuo Zhang; Caixia Xu; Qin Wei; Bin Du; Ru Li; Tianguo Zhang; Dan Wu; Yuxue Dai
Abstract
Nano-scale hydroxyapatite (HAp) was prepared by combining co-precipitation with microemulsion method, which exhibited strong adsorption for DNA due to its excellent biocompatibility and particular adsorbability. DNA and HAp could be modified onto glassy carbon electrode (GCE) by the simple and convenient ...
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Nano-scale hydroxyapatite (HAp) was prepared by combining co-precipitation with microemulsion method, which exhibited strong adsorption for DNA due to its excellent biocompatibility and particular adsorbability. DNA and HAp could be modified onto glassy carbon electrode (GCE) by the simple and convenient “tip-coating” method. Cyclic voltammetry was used to investigate the interaction of DNA immobilized on the HAp film with vitamin B12 (VB12). The existence of DNA led to the decrease of reduction current of VB12. Both the electron transfer coefficient (α) and the standard rate constant (ks) were different obtained on GCE and dsDNA/HAp/GCE, which indicated the formation of an electrochemical inactive super molecular complex DNA-nVB 12 . The equilibrium constant of this complex was calculated to be 5.35 × 10 5 mol·L -1 and the binding number between DNA and VB12 of the complex were determined to be one.
