Document Type : Research Article
Authors
- Manisha Bawskar 1
- Sunita Bansod 1
- Dnyaneshwar Rathod 1
- Carolina Alves dos Santos 2
- Pramod Ingle 1
- Mahendra Rai 1
- Aniket Gade 1
1 Nanobiotechnology Lab, Department of Biotechnology, Sant Gadge Baba Amravati University, Maharashtra, Amravati 444602, India
2 LaBNUS – Biomaterials and Nanotechnology Laboratory, University of Sorocaba, Sorocaba/SP, Brazil
Abstract
Fungal infections are major issue in agricultural crop plants that affects the growth of plant causing huge economic losses. Silver nanoparticles (AgNPs) have shown antimicrobial effects. Here, we report the potential application of phytostabilized AgNPs as a novel nanofungicide and growth promoter in Vigna radiata (L.) R. Wilczek (Mung). The aqueous extract of Azadirachta indica (Neem) challenged with AgNO3 (1mM) develops a brown colour, indicating AgNPs synthesis. Detection of AgNPs was monitored by UV-Vis spectroscopy, which showed peak at 415 nm. Further confirmation was done by Fourier Transform Infra-Red Spectroscopy which illustrates functional groups present in the capping proteins. Nanoparticle Tracking Analysis, and Transmission Electron Microscopy confirmed the synthesis of spherical, polydispersed nanoparticles in the range of 15-35 nm. AgNPs proved to exhibit antifungal potential in suppression of fungal plant pathogens. Seed germination percentage was much higher for the AgNPs treated seeds as compared to control. Handy-PEA analyzer (measures chl-a fluorescence) indicating seedlings inoculated with AgNPs were grown as the healthy plant with greater vitality, compared to control plants. Phytostabilized AgNPs were found to be biocompatible and have a promising attribute in developing a potent nanofungicide to enhance productivity rate and prevent fungal infections in crops with no potential toxicity.
Keywords
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