Nanofibers fabrication is generating considerable interest in terms of their biomedical applications. Recent development in nanofibers fabrication techniques resulted in controlled manipulation of nanofibers characteristics, such as their high surface to volume ratio, high porosity and their ability to encapsulate bioactive molecules. Development of biocompatible, polymer coated nanofibers can also provide an optimal environment for cell adhesion, proliferation and differentiation. This paper presents an overview on different applied techniques for nanofibers fabrication, in addition to the process variables to tailor their physico-chemical characteristics. Furthermore, the current review sheds a new light on the application of nanofibers on treatment of diabetic foot ulcers and artificial skin reconstruction.