A voltammetric sensor was developed for detection of glucose by using cyclic voltammetry (CV). The sensing platform was Polypyrrole-Pt nanocomposites on Platinum electrode (PPy-Pt-PtE). PPy-Pt was synthesized by chemical method, using FeCl3 oxidant. XRD, SEM and TEM results showed that PPy doped with Pt were highly porous, nanocrystalline composites. The PPy-Pt-PtE modified electrode observed reversible behavior with ferricyanide system which had about 2.05 times more surface area and exhibited higher currents for glucose oxidation compared to bare PtE. Glucose was sensed in the range of 100mM to 1000 mM from the linear regression plotted R 2 = 0.990 and R 2 = 0.994 the sensitivity was found to be 0.047 mA/mM~cm 2 and 0.0445 mA/mM~cm 2 . These results indicate that PPy-Pt-PtE exhibited good platform and could be used for voltammetric determination of glucose.