In recent decades bone infection is one of the most challenging issues encountered in biomedical field and local antibiotic delivery is a key strategy to overcome this issue. Hence developing bioactive materials in combination with antibiotics is much focused recently for bone substitutes. Here we report the fabrication of pristine and natural polymer (gelatin) composite matrices of hydroxyapatite (HA) by a facile wet precipitation method and their drug release behavior from directly loaded and in situ loaded matrices using amoxicillin as the model drug. The products thus obtained were analyzed by X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectroscopy, thermogravimetry (TG) and scanning electron microscopy (SEM) which confirmed the formation of HA and nanocomposite of HA with gelatin. It was observed that under physiological conditions, for sustained and prolonged release of the drug in situ loading in composite matrix is a favorable approach.