Gold nanoparticles (AuNPs) have found widespread applications in life sciences. Gold nanoparticles are of interest because of the unique properties which can be incorporated into cancer therapy applications, biosensor materials, composite fibers, cryogenic superconducting materials, cosmetic products, and electronic components. In the present research program, cost effective and environment friendly gold nanoparticles were synthesized using red grape pomace (GPM) ethanolic extract as a reducing agent and a capping agent. The nanoparticles were characterized using UV-visble, XRD, TEM and DLS methods. The absorption peak at 554 nm was found to be broadening with increase in time indicating the polydispersity nature of the nanoparticles. The XRD results suggested that the crystallization of the bioorganic phase occurs on the surface of the gold nanoparticles or vice versa. The TEM image showed relatively spherical shape nanoparticles. We also Purified anti-OMP85 antibody were successfully conjugated on 13 ± 34 nm gold nanoparticles by an electrostatic adsorption method.