AnchorPlant mediated synthesis of metallic nanoparticles is an increasing commercial demand due to the wide applicability in various areas such as electronics, catalysis, chemistry, energy, cosmetics and medicine. In the present investigation, synthesis of gold nanoparticles is done by using fruit extracts of Ananas comosus (L.). Nanoparticles were characterized by using UV visible absorption spectra. Their morphology, elemental composition and crystalline phase were determined by scanning electron microscopy, energy dispersive X-ray spectroscopy and selected area electron diffraction. FT-IR analysis was used to confirm the presence of gold nanoparticles in the extracts. The synthesized gold nanoparticles were generally found to be effective as antimicrobial agents against some important human pathogens like E.coli and Streptobacillus sp. which are affecting and cause diseases like food poisoning and rat-bite fever to human beings respectively.