We have electrochemically synthesized polyaniline nano fibers with optimized process parameters (viz. concentration of monomer and dopant, applied current density, deposition time, etc.) on ITO coated glass substrate. The nano fibers of polyaniline were subjected to UV Visible, SEM and Raman spectroscopy. UV Vis spectra show two prominent peaks at 317 and 418 nm, which confirm the presence of different forms of polyaniline. Raman spectra confirm the formation of polyaniline. SEM image of synthesized nano fibers showed a flower like structure with an isotropic growth rate.