Seetharamaiah Nalini; Seetharamaiah Nandini; Shivayogeeswar E. Neelagund; Jose Savio Melo; Gurukar Shivappa Suresh
Abstract
In this report, extractions of the Calotropis procera polyphenols have been carried out in conventional soxhlet assisted extraction (SAE) and it is compared with microwave assisted extraction (MAE). As an extension of our previous work reported, we attempted to study the electrochemical interaction of ...
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In this report, extractions of the Calotropis procera polyphenols have been carried out in conventional soxhlet assisted extraction (SAE) and it is compared with microwave assisted extraction (MAE). As an extension of our previous work reported, we attempted to study the electrochemical interaction of the aqueous plant extract with DNA modified electrode Gr/PAH/NT-G/PPy/DNA. The binding constant Kf determined using UV-Vis spectroscopy was found to be 4.91×10 -1 and 4.55×10 -1 µmol dm -3 for soxhlet assisted plant extract (SAPE) and microwave assisted plant extract (MAPE) respectively. This was consistent with the electrochemical determination of Kf 4.31×10 -6 and 4.71×10 -4 µmol dm -3 for SAPE and MAPE respectively. Further the surface coverage area (Γs) of the electrode fabricated using DNA was calculated using Chronocoulometry for the plant extracts. The linear range of the aqueous extract on the Gr/PAH/NT-G/PPy/DNA modified electrode was found to be from 1-250 µmol dm -3 , with a limit of detection of 0.22 and 0.29 µmol dm -3 mL -1 for SAPE and MAPE respectively. The limit of quantification was found to be 0.68 and 0.88 µmol dm -3 mL -1 for SAPE and MAPE respectively. As a result Calotropis procera can be studied further to applied for anticancer activity. Moreover, electrochemical methods can be a promising technique in drug analysis.
Seetharamaiah Nalini; Seetharamaiah Nandini; Gurukar Shivappa Suresh; Jose Savio Melo; Shivayogeeswar E. Neelagund; Hunasepalya Nagaiah Naveen Kumar; Jakkid Sanetuntikul; Sangaraju Shanmugam
Abstract
In this article, we report the pre-screening of anti-cancer effect of Calotropis procera against glioblastoma cell lines (LN-18) by means of electrochemical methods. Soxhlet assisted extraction (SAE) has been employed to extract the polyphenol contents present in the leaf of Calotropis procera. The phytochemical ...
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In this article, we report the pre-screening of anti-cancer effect of Calotropis procera against glioblastoma cell lines (LN-18) by means of electrochemical methods. Soxhlet assisted extraction (SAE) has been employed to extract the polyphenol contents present in the leaf of Calotropis procera. The phytochemical analysis of the extract has been studied and the polyphenol contents were determined using Folin Ciocalteau method. To study the anticancer effects of the aqueous plant extract, a cytosensor (Gr/NT-G/LN-18) was fabricated and its possible mechanism for DNA binding was studied using graphite/ poly(allylamine hydrochloride)/nanotube-graphene composite /polypyrrole /de-oxy ribonucleic acid (Gr/PAH/NT-G/PPy/DNA) modified electrode. The electrochemical characteristics of the proposed Gr/NT-G/LN-18 cytosensor towards the plant extract were evaluated using electrochemical techniques like cyclic voltammetry and differential pulse voltammetry. Scanning electron microscopy (SEM) and energy dispersive analysis of x-ray (EDAX) have been employed to study the physical characterization of the Gr/PAH/NT-G/PPy/DNA modified electrode. These results indicate that the plant extract has an ability to act as an antiglioblastoma against LN-18 cancer cells.