Dasal Vasantharaja; Venugopal Ramalingam; Shanmugam Thangapandiyan; Nagarajan Sridhar; Gaddam Dayanand Reddy
Abstract
Nanoparticles (NPs) are extensively being used in modern life due to their distinctive properties like small size having large surface area. Titanium dioxide (TiO2) NPs are mostly used in cosmetic products, food additives, pharmaceuticals and electronics. They are capable of inducing oxidative stress ...
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Nanoparticles (NPs) are extensively being used in modern life due to their distinctive properties like small size having large surface area. Titanium dioxide (TiO2) NPs are mostly used in cosmetic products, food additives, pharmaceuticals and electronics. They are capable of inducing oxidative stress in both animals and human. The intention of this study was to find out the hepatotoxic effect of TiO2 NPs on the male Wistar rats. The animals were divided in to three groups. First group received normal saline; Second group received TiO2 NPs (50 mg/kg/bw) for 14 days continuously, while third group received TiO2 NPs (100 mg/kg/bw) for the same duration. The increased levels of specific markers AST, ALT, ALP, LDH and GGT along with the TBARS, LOOH, CD and PC in the liver clearly shows the hepatotoxic action of TiO2. The hepatotoxic nature of TiO2 NPs was more evidenced by the diminished activity of antioxidant enzymes levels and also showed augmented DNA damage and fragmentation in hepatocytes. In conclusion, the data indicated that TiO2 NPs induced oxidative stress which produces hepatotoxicity in the rat liver.
Ranu K. Dutta; Prashant K. Sharma; Avinash C. Pandey
Abstract
Here is an insight into the effects of interaction of ZnO nanoparticles and the various cellular level changes that are brought about by the help of Raman spectroscopy on individual Escherichia coli cells. Raman vibrational signatures show variation in peak intensities of some of the cellular components ...
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Here is an insight into the effects of interaction of ZnO nanoparticles and the various cellular level changes that are brought about by the help of Raman spectroscopy on individual Escherichia coli cells. Raman vibrational signatures show variation in peak intensities of some of the cellular components of E coli cells with increase in nanoparticles concentration. This can be attributed to the cellular and molecular changes associated with bacterial cell growth, as the cells proceed from lag phase to stationary phase, which indicates that ZnO interferences with bacterial growth. Growth kinetics studies show mitigation in growth and colony forming units (CFU) counts. Changes in cellular morphology as investigated by atomic force microscopy and scanning electron microscopy, show destruction and even rupture of cell wall at higher ZnO concentration. This study pertains to any alterations brought about at the cellular level, which may be extended to other nanomaterials in the environment and the effect on human cells as well.