Ardeshir Shokrollahi; Samira Hessampour
Abstract
In this paper, the Taguchi orthogonal design was applied to optimize the extraction efficiencies for the determination of curcumin, after the separation and preconcenteration by supramolecular based-ultrasonic assisted-dispersion solidification liquid-liquid microextraction (SM-UA-DSLLME) procedure coupled ...
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In this paper, the Taguchi orthogonal design was applied to optimize the extraction efficiencies for the determination of curcumin, after the separation and preconcenteration by supramolecular based-ultrasonic assisted-dispersion solidification liquid-liquid microextraction (SM-UA-DSLLME) procedure coupled with spectrophotometric UV-Vis. The experimental design consisted of six parameters (pH, amount of the extraction and disperser solvents, salt effect, sonication time and centrifuge time), each at five levels. The preconcentration method is based on the dispersion-solidification liquid-liquid microextraction of curcumin with decanoic acid/THF as the supramolecular solvent (a nano-structured liquid occurring on two scales, molecular and nano). The purpose method had two linear ranges of 0.01–0.40 and 0.40-3.50 mg L−1 of curcumin with R2= 0.9922 and 0.9799, respectively. The enrichment factors of 478.48 and 118.80 were obtained for down and up linear ranges, respectively. The preconcentration factor under consideration recovery was obtained 46. Detection limit was 5.2 µg L−1 and the relative standard deviation (RSD%), for eight replicate measurements of 0.2 mg L−1 curcumin was 2.47%. The results obtained from the analysis of variance (ANOVA), revealed that the most important effectible parameters onextraction curcumin are volume of disperser solvent and pH. The applicability of method was successfully applied to determine of trace curcumin in tablet, sewage and water samples.
Ganeshlenin Kandasamy; Atul Sudame; Dipak Maity
Abstract
In this work, we have synthesized oleylamine (OM)-coated hydrophobic monodispersed SPIONs with an average particle size of ~9 nm via thermal decomposition method. The as-prepared hydrophobic SPIONs are co-encapsulated along with a drug (curcumin, Cur) within the mixed micelles based nanoformulations ...
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In this work, we have synthesized oleylamine (OM)-coated hydrophobic monodispersed SPIONs with an average particle size of ~9 nm via thermal decomposition method. The as-prepared hydrophobic SPIONs are co-encapsulated along with a drug (curcumin, Cur) within the mixed micelles based nanoformulations which is made of d-α-Tocopheryl polyethylene glycol 1000 succinate (TPGS) and Pluronic F127 while keeping the TPGS:F127 ratios at 100:0, 75:25, 50:50, 25:75 and 0:100. Then, the nanoformulations are characterized for hydrodynamic size via dynamic light scattering (DLS) technique, and drug/SPIONs encapsulation efficiencies are determined via UV-vis spectroscopy. Among all the nanoformulation, the mixed micelle with 50:50 TPGS:F127 has exhibited relatively lower hydrodynamic diameter (Dh) (~ 84 nm), better encapsulation efficiencies of Cur and SPIONs (~95% / 56%), and high yield (above 90%). Moreover, morphology and encapsulation of SPIONs/Cur inside the optimized 50:50 TPGS:F127 nanoformulation is confirmed by TEM. In addition, only 10% of Cur is released during 12h time period from optimized nanoformulation indicating the sustained-release property, whereas ~68% of Cur is quickly released in free Cur experiments for the same time period. Hence, the SPIONs/Cur are efficiently co-encapsulated inside the TPGS:F127 mixed micelle based nanoformulation which could be used for further biomedical applications.